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Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

TitleBirth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
Publication TypeJournal Article
Year of Publication2018
AuthorsCrozet, P, Navarro, FJ, Willmund, F, Mehrshahi, P, Bakowski, K, Lauersen, KJ, Perez-Perez, ME, Auroy, P, A. Rovira, G, Sauret-Gueto, S, Niemeyer, J, Spaniol, B, Theis, J, Trosch, R, Westrich, LD, Vavitsas, K, Baier, T, Hubner, W, de Carpentier, F, Cassarini, M, Danon, A, Henri, J, Marchand, CH, De Mia, M, Sarkissian, K, Baulcombe, DC, Peltier, G, Crespo, JL, Kruse, O, Jensen, PE, Schroda, M, Smith, AG, Lemaire, SD
JournalACS Synth Biol
Volume7
Pagination2074-2086
Date PublishedSep 21
ISBN Number2161-5063 (Electronic)2161-5063 (Linking)
Keywords*Photosynthesis, Biotechnology, Chlamydomonas reinhardtii/genetics/*metabolism, Gene Expression, Genes, Reporter/genetics, Green Fluorescent Proteins/genetics/metabolism, Plasmids/genetics/*metabolism, Promoter Regions, Genetic, Synthetic Biology/*methods
Abstract

Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/30165733
Short TitleACS synthetic biology