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bz-rates: A Web Tool to Estimate Mutation Rates from Fluctuation Analysis

Titlebz-rates: A Web Tool to Estimate Mutation Rates from Fluctuation Analysis
Publication TypeJournal Article
Year of Publication2015
AuthorsGillet-Markowska, A, Louvel, G, Fischer, G
JournalG3: Genes|Genomes|Genetics
Abstract

Fluctuation analyses is the standard experimental method for measuring mutation rates in microorganisms. The appearance of mutants is classically described by a Luria-Delbrück distribution composed of two parameters: the number of mutations per culture (m) and the differential growth rate between mutant and wild-type cells (b). A precise estimation of these 2 parameters is a prerequisite to the calculation of the mutation rate. Here, we developed bz-rates, a web tool to calculate mutation rates that provides three useful advances over existing web tools. First, it allows taking into account b, the differential growth rate between mutant and wild-type cells, in the estimation of m with the Generating Function (GF). Secondly, bz-rates allows the user to take into account a deviation from the Luria-Delbrück distribution called z, the plating efficiency, in the estimation of m. Finally, the website provides a graphical visualization of the goodness-of-fit between the experimental data and the model. bz-rates is accessible at http://www.lcqb.upmc.fr/bzrates.

URLhttp://www.g3journal.org/content/early/2015/09/02/g3.115.019836.abstract
DOI10.1534/g3.115.019836