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The central role of PDR1 in the foundation of yeast drug resistance.

TitleThe central role of PDR1 in the foundation of yeast drug resistance.
Publication TypeJournal Article
Year of Publication2007
AuthorsFardeau, V, Lelandais, G, Oldfield, A, Salin, H, Lemoine, S, Garcia, M, Tanty, V, Le Crom, S, Jacq, C, Devaux, F
JournalJ Biol Chem
Volume282
Issue7
Pagination5063-74
Date Published2007 Feb 16
ISSN0021-9258
KeywordsAntipsychotic Agents, Benomyl, DNA-Binding Proteins, Drug Resistance, Fungal, Fluphenazine, Fungicides, Industrial, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors, Transcription, Genetic
Abstract

The widespread pleiotropic drug resistance (PDR) phenomenon is well described as the long term selection of genetic variants expressing constitutively high levels of membrane transporters involved in drug efflux. However, the transcriptional cascades leading to the PDR phenotype in wild-type cells are largely unknown, and the first steps of this phenomenon are poorly understood. We investigated the transcriptional mechanisms underlying the establishment of an efficient PDR response in budding yeast. We show that within a few minutes of drug sensing yeast elicits an effective PDR response, involving tens of PDR genes. This early PDR response (ePDR) is highly dependent on the Pdr1p transcription factor, which is also one of the major genetic determinants of long term PDR acquisition. The activity of Pdr1p in early drug response is not drug-specific, as two chemically unrelated drugs, benomyl and fluphenazine, elicit identical, Pdr1p-dependent, ePDR patterns. Our data also demonstrate that Pdr1p is an original stress response factor, the DNA binding properties of which do not depend on the presence of drugs. Thus, Pdr1p is a promoter-resident regulator involved in both basal expression and rapid drug-dependent induction of PDR genes.

DOI10.1074/jbc.M610197200
Alternate JournalJ. Biol. Chem.
PubMed ID17158869