You are here

Competitive promoter occupancy by two yeast paralogous transcription factors controlling the multidrug resistance phenomenon.

TitleCompetitive promoter occupancy by two yeast paralogous transcription factors controlling the multidrug resistance phenomenon.
Publication TypeJournal Article
Year of Publication2003
AuthorsLucau-Danila, A, Delaveau, T, Lelandais, G, Devaux, F, Jacq, C
JournalJ Biol Chem
Volume278
Issue52
Pagination52641-50
Date Published2003 Dec 26
ISSN0021-9258
KeywordsAlleles, Blotting, Northern, Cell Membrane, Chromatin, DNA, Drug Resistance, Genome, Fungal, Models, Biological, Mutation, Oligonucleotide Array Sequence Analysis, Plasmids, Precipitin Tests, Promoter Regions, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors, Transcription Factors, Transcription, Genetic, Up-Regulation, Zinc Fingers
Abstract

Highly flexible gene expression programs are required to allow cell growth in the presence of a wide variety of chemicals. We used genome-wide expression analyses coupled with chromatin immunoprecipitation experiments to study the regulatory relationships between two very similar yeast transcription factors involved in the control of the multidrug resistance phenomenon. Yrm1 (Yor172w) is a new zinc finger transcription factor, the overproduction of which decreases the level of transcription of the target genes of Yrr1, a zinc finger transcription factor controlling the expression of several membrane transporter-encoding genes. Surprisingly, the absence of YRR1 releases the transcriptional activity of Yrm1, which then up-regulates 23 genes, 14 of which are also direct target genes of Yrr1. Chromatin immunoprecipitation experiments confirmed that Yrm1 binds to the promoters of the up-regulated genes only in yeast strains from which YRR1 has been deleted. This sophisticated regulatory program can be associated with drug resistance phenotypes of the cell. The program-specific distribution of paired transcription factors throughout the genome may be a general mechanism by which similar transcription factors regulate overlapping gene expression programs in response to chemical stress.

DOI10.1074/jbc.M309580200
Alternate JournalJ. Biol. Chem.
PubMed ID14512416