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Detection of IgG directed against a recombinant form of Epstein-Barr virus BALF0/1 protein in patients with nasopharyngeal carcinoma

TitleDetection of IgG directed against a recombinant form of Epstein-Barr virus BALF0/1 protein in patients with nasopharyngeal carcinoma
Publication TypeJournal Article
Year of Publication2019
AuthorsShao, Z, Borde, C, Marchand, CH, Lemaire, SD, Busson, P, Gozlan, JM, Escargueil, A, Marechal, V
JournalProtein Expr Purif
Volume162
Pagination44-50
Date PublishedOct
ISBN Number1096-0279 (Electronic)1046-5928 (Linking)
Abstract

BALF0/1 is a putative Epstein-Barr virus (EBV) protein that has been described as a modulator of apoptosis. So far, the lack of specific immunological reagents impaired the detection of native BALF0/1 in EBV-infected cells. This study describes the expression and purification of a truncated form of BALF0/1 (tBALF0) using a heterologous bacterial expression system. tBALF0 was further used as an antigen in an indirect Enzyme-linked Immunosorbent Assay (ELISA) that unraveled the presence of low titer IgGs to BALF0/1 during primary (10.0%) and past (13.3%) EBV infection. Conversely high-titer IgGs to BALF0/1 were detected in 33.3% of nasopharyngeal carcinoma (NPC) patients suggesting that BALF0/1 and/or humoral response against it may contribute to NPC pathogenesis.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/31145974
Short TitleProtein expression and purification