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End-to-end fusion of linear deleted chromosomes initiates a cycle of genome instability in Streptomyces ambofaciens.

TitleEnd-to-end fusion of linear deleted chromosomes initiates a cycle of genome instability in Streptomyces ambofaciens.
Publication TypeJournal Article
Year of Publication2003
AuthorsWenner, T, Roth, V, Fischer, G, Fourrier, C, Aigle, B, Decaris, B, Leblond, P
JournalMol Microbiol
Volume50
Issue2
Pagination411-25
Date Published2003 Oct
ISSN0950-382X
KeywordsChromosomes, Bacterial, DNA, Bacterial, Gene Rearrangement, Genome, Bacterial, Genomic Instability, Mutation, Recombination, Genetic, Streptomyces
Abstract

Two mutant strains harbouring a linear chromosome whose size reached 13 Mb (versus approximately 8 Mb for the wild type) were characterized. This chromosomal structure resulted from the fusion in inverted orientation of two chromosomes partially deleted on the same arm. The fusion occurred by illegitimate recombination between 6 bp repeats. This chromosomal structure was inherited in strict association with a high level of genetic instability (30% of mutants in a single progeny, phenomenon also called hypervariability) and chromosomal instability. In contrast, derivatives, which did not retain the chromosome fusion, showed a wild-type-like instability frequency (c. 1%). Stabilization of the chromosomal structure occurred by chromosome arm replacement or circularization. A high variability of the terminal inverted repeat (TIR) length in the rescued chromosomes (from 5 kb to approximately 1.4 Mb for linear derivatives) was observed. Mutant lineages harbouring the chromosomal fusion are characterized by a highly heterogeneous distribution of DNA in the spores, by the presence of spores without DNA as well as aberrant sporulation figures, and by the production of spores with a low germination rate. The wild-type characteristics were restored in the descendants, which lost the chromosomal fusion. Thus, the fusion of deleted chromosomes initiates a cycle of chromosome instability sharing several levels of analogy with the behaviour of dicentric chromosomes in eukaryotes. We propose that the high instability of the fused chromosomes results from the duplication of a region involved in partitioning of the chromosomes (parAB-oriC ).

Alternate JournalMol. Microbiol.
PubMed ID14617168