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Following the folding of RNA with time-resolved synchrotron X-ray footprinting.

TitleFollowing the folding of RNA with time-resolved synchrotron X-ray footprinting.
Publication TypeJournal Article
Year of Publication1998
AuthorsSclavi, B, Woodson, S, Sullivan, M, Chance, M, Brenowitz, M
JournalMethods Enzymol
Volume295
Pagination379-402
Date Published1998
ISSN0076-6879
KeywordsFlow Injection Analysis, Hydroxyl Radical, Kinetics, Ligands, Magnesium, Nucleic Acid Conformation, RNA, Synchrotrons, X-Rays
Abstract

The rapid mixing synchrotron X-ray footprinting technique described in this article allows nucleic acid folding and ligand binding reactions to be followed on a millisecond time resolution with single nucleotide resolution. In principle, the change in .OH protection of every nucleotide in a nucleic acid hundreds of nucleotides long can be monitored separately. In addition, a wide range of solution conditions are compatible with the radiolytic generation of .OH. These characteristics of synchrotron X-ray footprinting create opportunities for conducting thermodynamic and kinetic studies of nucleic acids that are both comprehensive and detailed. Kinetic footprinting studies of a number of systems have been initiated by the Center for Synchrotron Biosciences using this technique.

Alternate JournalMeth. Enzymol.
PubMed ID9750229