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Gene clusters reflecting macrodomain structure respond to nucleoid perturbations.

TitleGene clusters reflecting macrodomain structure respond to nucleoid perturbations.
Publication TypeJournal Article
Year of Publication2011
AuthorsScolari, VF, Bassetti, B, Sclavi, B, Cosentino Lagomarsino, M
JournalMol Biosyst
Volume7
Issue3
Pagination878-88
Date Published2011 Mar
ISSN1742-2051
KeywordsBacterial Proteins, Biofilms, DNA, Bacterial, DNA, Superhelical, DNA-Binding Proteins, Escherichia coli Proteins, Factor For Inversion Stimulation Protein, Gene Deletion, Genome, Bacterial, Multigene Family, Nucleic Acid Conformation, Oligonucleotide Array Sequence Analysis, Protein Conformation, Transcription, Genetic
Abstract

Focusing on the DNA-bridging nucleoid proteins Fis and H-NS, and integrating several independent experimental and bioinformatic data sources, we investigate the links between chromosomal spatial organization and global transcriptional regulation. By means of a novel multi-scale spatial aggregation analysis, we uncover the existence of contiguous clusters of nucleoid-perturbation sensitive genes along the genome, whose expression is affected by a combination of topological DNA state and nucleoid-shaping protein occupancy. The clusters correlate well with the macrodomain structure of the genome. The most significant of them lay symmetrically at the edges of the Ter macrodomain and involve all of the flagellar and chemotaxis machinery, in addition to key regulators of biofilm formation, suggesting that the regulation of the physical state of the chromosome by the nucleoid proteins plays an important role in coordinating the transcriptional response leading to the switch between a motile and a biofilm lifestyle.

DOI10.1039/c0mb00213e
Alternate JournalMol Biosyst
PubMed ID21165487