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Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology.

TitleGenome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology.
Publication TypeJournal Article
Year of Publication2014
AuthorsDaboussi, F, Leduc, S, Maréchal, A, Dubois, G, Guyot, V, Perez-Michaut, C, Amato, A, Falciatore, A, Juillerat, A, Beurdeley, M, Voytas, DF, Cavarec, L, Duchateau, P
JournalNat Commun
Volume5
Pagination3831
Date Published2014
ISSN2041-1723
Abstract

Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.

DOI10.1038/ncomms4831
Alternate JournalNat Commun
PubMed ID24871200