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Intraclonal polymorphism in the bacterium Streptomyces ambofaciens ATCC23877: evidence for a high degree of heterogeneity of the wild type clones.

TitleIntraclonal polymorphism in the bacterium Streptomyces ambofaciens ATCC23877: evidence for a high degree of heterogeneity of the wild type clones.
Publication TypeJournal Article
Year of Publication1999
AuthorsMartin, P, Dary, A, André, A, Fischer, G, Leblond, P, Decaris, B
JournalMutat Res
Volume430
Issue1
Pagination75-85
Date Published1999 Nov 29
ISSN0027-5107
KeywordsChromosomes, Bacterial, Cosmids, DNA Probes, DNA, Recombinant, Genetic Heterogeneity, Genetic Markers, Pigments, Biological, Polymorphism, Genetic, Sequence Deletion, Streptomyces
Abstract

In Streptomyces ambofaciens a genetic instability generates a high degree of polymorphism consisting of four main phenotypes: pigmented colonies (Pig(+) qualified as WT phenotype), pigment-defective colonies, pigmented colonies with pigment-defective sector and pigmented colonies with pigment-defective papillae. Molecular analysis of Pig(col)(-) and Pig(sec)(-) (pigment-defective mutant derived from a colony and a sector, respectively) produced by genetic instability and isolated in five Pig(+) subclones progenies revealed a new aspect of polymorphism in S. ambofaciens ATCC23877. Frequencies of Pig(col)(-) and Pig(sec)(-) mutants deleted at the chromosome ends varied from one WT progeny to another. Two main types of deleted mutants were observed: deleted for one or both chromosomal extremities. The relative proportion of these two categories differed according to the WT progeny. These results argue for heterogeneity of the WT clones, i.e., Pig(+) colonies, originated from S. ambofaciens ATCC23877.

Alternate JournalMutat. Res.
PubMed ID10592319