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Large scale chromosomal mapping of human microRNA structural clusters.

TitleLarge scale chromosomal mapping of human microRNA structural clusters.
Publication TypeJournal Article
Year of Publication2013
AuthorsMathelier, A, Carbone, A
JournalNucleic Acids Res
Volume41
Issue8
Pagination4392-408
Date Published2013 Apr
ISSN1362-4962
Keywords3' Untranslated Regions, Algorithms, Chromosome Fragile Sites, Chromosome Mapping, Chromosomes, Human, High-Throughput Nucleotide Sequencing, Humans, MicroRNAs, Neoplasms, Nucleic Acid Conformation
Abstract

MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes crossing sequence analysis and deep sequencing data revealed the presence of >400 structural clusters of miRNAs in the human genome. An a posteriori analysis validates predictions as bona fide miRNAs. A functional analysis of structural clusters position along the chromosomes co-localizes them with genes involved in several key cellular processes like immune systems, sensory systems, signal transduction and development. Immune systems diseases, infectious diseases and neurodegenerative diseases are characterized by genes that are especially well organized around structural clusters of miRNAs. Target genes functional analysis strongly supports a regulatory role of most predicted miRNAs and, notably, a strong involvement of predicted miRNAs in the regulation of cancer pathways. This analysis provides new fundamental insights on the genomic organization of miRNAs in human chromosomes.

DOI10.1093/nar/gkt112
Alternate JournalNucleic Acids Res.
PubMed ID23444140
PubMed Central IDPMC3632110

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