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Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.

TitleLarge-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.
Publication TypeJournal Article
Year of Publication2004
AuthorsBoyer, J, Badis, G, Fairhead, C, Talla, E, Hantraye, F, Fabre, E, Fischer, G, Hennequin, C, Koszul, R, Lafontaine, I, Ozier-Kalogeropoulos, O, Ricchetti, M, Richard, G-F, Thierry, A, Dujon, B
JournalGenome Biol
Volume5
Issue9
PaginationR72
Date Published2004
ISSN1465-6914
KeywordsCloning, Molecular, DNA, Fungal, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Lethal, Genes, Suppressor, Genome, Fungal, Open Reading Frames, Phenotype, Reading Frames, Saccharomyces cerevisiae, Transfection, Transgenes
Abstract

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

DOI10.1186/gb-2004-5-9-r72
Alternate JournalGenome Biol.
PubMed ID15345056
PubMed Central IDPMC522879