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Mitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA.

TitleMitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA.
Publication TypeJournal Article
Year of Publication2010
AuthorsGarcia, M, Delaveau, T, Goussard, S, Jacq, C
JournalEMBO Rep
Volume11
Issue4
Pagination285-91
Date Published2010 Apr
ISSN1469-3178
KeywordsBiological Transport, Genome, Mitochondrial, In Situ Hybridization, Fluorescence, Mitochondria, Models, Biological, Open Reading Frames, Proton-Translocating ATPases, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Abstract

Although a considerable amount of data have been gathered on mitochondrial translocases, which control the import of a large number of nuclear-encoded proteins, the preceding steps taking place in the cytosol are poorly characterized. The localization of messenger RNAs (mRNAs) on the surface of mitochondria was recently shown to involve specific classes of protein and could be an important regulatory step. By using an improved statistical fluorescent in situ hybridization technique, we analysed the elements of the ATP2 open reading frame that control its mRNA asymmetric localization. The amino-terminal mitochondrial targeting peptide (MTS) and translation of two elements in the coding sequence, R1 and R2, were required for anchoring of ATP2 mRNA to mitochondria. Unexpectedly, any MTS can replace ATP2 MTS, whereas R1 and R2 are specifically required to maintain perimitochondrial mRNA localization. These data connect the well-known MTS-translocase interaction step with a site-specific translation step and offer a mechanistic description for a co-translational import process.

DOI10.1038/embor.2010.17
Alternate JournalEMBO Rep.
PubMed ID20224577
PubMed Central IDPMC2854591