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From principal component to direct coupling analysis of coevolution in proteins: low-eigenvalue modes are needed for structure prediction.

TitleFrom principal component to direct coupling analysis of coevolution in proteins: low-eigenvalue modes are needed for structure prediction.
Publication TypeJournal Article
Year of Publication2013
AuthorsCocco, S, Monasson, R, Weigt, M
JournalPLoS Comput Biol
Volume9
Issue8
Paginatione1003176
Date Published2013
ISSN1553-7358
KeywordsAlgorithms, Entropy, Models, Molecular, Principal Component Analysis, Protein Conformation, Proteins, Sequence Alignment, Sequence Analysis, Protein
Abstract

Various approaches have explored the covariation of residues in multiple-sequence alignments of homologous proteins to extract functional and structural information. Among those are principal component analysis (PCA), which identifies the most correlated groups of residues, and direct coupling analysis (DCA), a global inference method based on the maximum entropy principle, which aims at predicting residue-residue contacts. In this paper, inspired by the statistical physics of disordered systems, we introduce the Hopfield-Potts model to naturally interpolate between these two approaches. The Hopfield-Potts model allows us to identify relevant 'patterns' of residues from the knowledge of the eigenmodes and eigenvalues of the residue-residue correlation matrix. We show how the computation of such statistical patterns makes it possible to accurately predict residue-residue contacts with a much smaller number of parameters than DCA. This dimensional reduction allows us to avoid overfitting and to extract contact information from multiple-sequence alignments of reduced size. In addition, we show that low-eigenvalue correlation modes, discarded by PCA, are important to recover structural information: the corresponding patterns are highly localized, that is, they are concentrated in few sites, which we find to be in close contact in the three-dimensional protein fold.

DOI10.1371/journal.pcbi.1003176
Alternate JournalPLoS Comput. Biol.
PubMed ID23990764
PubMed Central IDPMC3749948

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