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Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications.

TitleRapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications.
Publication TypeJournal Article
Year of Publication2016
AuthorsSi, Y, Grazon, C, Clavier, G, Rieger, J, Audibert, J-F, Sclavi, B, Méallet-Renault, R
JournalBiosens Bioelectron
Volume75
Pagination320-7
Date Published2016 Jan 15
ISSN1873-4235
KeywordsBiosensing Techniques, Escherichia coli, Fluorescence, High-Throughput Screening Assays, Hydrogen-Ion Concentration, Nanoparticles
Abstract

Rapid detection of bacterial growth is an important issue in the food industry and for medical research. Here we present a novel kind of pH-sensitive fluorescent nanoparticles (FANPs) that can be used for the rapid and accurate real-time detection of Escherichia coli growth. These organic particles are designed to be non-toxic and highly water-soluble. Here we show that the coupling of pH sensitive fluoresceinamine to the nanoparticles results in an increased sensitivity to changes in pH within a physiologically relevant range that can be used to monitor the presence of live bacteria. In addition, these FANPs do not influence bacterial growth and are stable over several hours in a complex medium and in the presence of bacteria. The use of these FANPs allows for continuous monitoring of bacterial growth via real-time detection over long time scales in small volumes and can thus be used for the screening of a large number of samples for high-throughput applications such as screening for the presence of antibiotic resistant strains.

DOI10.1016/j.bios.2015.08.028
Alternate JournalBiosens Bioelectron
PubMed ID26334591