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RNA folding at millisecond intervals by synchrotron hydroxyl radical footprinting.

TitleRNA folding at millisecond intervals by synchrotron hydroxyl radical footprinting.
Publication TypeJournal Article
Year of Publication1998
AuthorsSclavi, B, Sullivan, M, Chance, MR, Brenowitz, M, Woodson, SA
JournalScience
Volume279
Issue5358
Pagination1940-3
Date Published1998 Mar 20
ISSN0036-8075
KeywordsAnimals, Hydroxyl Radical, Kinetics, Magnesium, Models, Molecular, Nucleic Acid Conformation, RNA, Catalytic, Solvents, Synchrotrons, Tetrahymena, X-Rays
Abstract

Radiolysis of water with a synchrotron x-ray beam permits the hydroxyl radical-accessible surface of an RNA to be mapped with nucleotide resolution in 10 milliseconds. Application of this method to folding of the Tetrahymena ribozyme revealed that the most stable domain of the tertiary structure, P4-P6, formed cooperatively within 3 seconds. Exterior helices became protected from hydroxyl radicals in 10 seconds, whereas the catalytic center required minutes to be completely folded. The results show that rapid collapse to a partially disordered state is followed by a slow search for the active structure.

Alternate JournalScience
PubMed ID9506944
Grant ListGM39929 / GM / NIGMS NIH HHS / United States
GM51506 / GM / NIGMS NIH HHS / United States
GM52348 / GM / NIGMS NIH HHS / United States