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Short-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization.

TitleShort-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization.
Publication TypeJournal Article
Year of Publication2013
AuthorsJaver, A, Long, Z, Nugent, E, Grisi, M, Siriwatwetchakul, K, Dorfman, KD, Cicuta, P, Cosentino Lagomarsino, M
JournalNat Commun
Volume4
Pagination3003
Date Published2013
ISSN2041-1723
KeywordsChromosomes, Bacterial, Diffusion, Escherichia coli, Genetic Loci, Movement, Subcellular Fractions, Time Factors
Abstract

In bacteria, chromosomal architecture shows strong spatial and temporal organization, and regulates key cellular functions, such as transcription. Tracking the motion of chromosomal loci at short timescales provides information related to both the physical state of the nucleo-protein complex and its local environment, independent of large-scale motions related to genome segregation. Here we investigate the short-time (0.1-10 s) dynamics of fluorescently labelled chromosomal loci in Escherichia coli at different growth rates. At these timescales, we observe for the first time a dependence of the loci's apparent diffusion on both their subcellular localization and chromosomal coordinate, and we provide evidence that the properties of the chromosome are similar in the tested growth conditions. Our results indicate that either non-equilibrium fluctuations due to enzyme activity or the organization of the genome as a polymer-protein complex vary as a function of the distance from the origin of replication.

DOI10.1038/ncomms3003
Alternate JournalNat Commun
PubMed ID23764719