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Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

TitleChemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis
Publication TypeJournal Article
Year of Publication2010
AuthorsYe, S, Berger, AAnn, Petzold, D, Reimann, O, Matt, B, Koksch, B
Secondary AuthorsO’Hagan, D
JournalBeilstein Journal of Organic Chemistry
Volume6
Pagination40
Date Published2010
ISBN Number1860-5397
Abstract

This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines’ interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.

URLhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2874332/
Short TitleBeilstein J Org Chem