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Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging.

TitleControl of Protein Activity and Gene Expression by Cyclofen-OH Uncaging.
Publication TypeJournal Article
Year of Publication2018
AuthorsZhang, W, Hamouri, F, Feng, Z, Aujard, I, Ducos, B, Ye, S, Weiss, S, Volovitch, M, Vriz, S, Jullien, L, Bensimon, D
JournalChembiochem
Volume19
Issue12
Pagination1232-1238
Date Published2018 Jun 18
ISSN1439-7633
Abstract

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.

DOI10.1002/cbic.201700630
Alternate JournalChembiochem
PubMed ID29341391