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Gene silencing and large-scale domain structure of the E. coli genome.

TitleGene silencing and large-scale domain structure of the E. coli genome.
Publication TypeJournal Article
Year of Publication2013
AuthorsZarei, M, Sclavi, B, Cosentino Lagomarsino, M
JournalMol Biosyst
Volume9
Issue4
Pagination758-67
Date Published2013 Apr 5
ISSN1742-2051
KeywordsBacterial Proteins, Binding Sites, Chromosome Mapping, DNA-Binding Proteins, Escherichia coli, Gene Expression Regulation, Bacterial, Gene Silencing, Genome, Bacterial, Protein Binding
Abstract

The H-NS chromosome-organizing protein in E. coli can stabilize genomic DNA loops, and form oligomeric structures connected to repression of gene expression. Motivated by the link between chromosome organization, protein binding and gene expression, we analyzed publicly available genomic data sets of various origins, from genome-wide protein binding profiles to evolutionary information, exploring the connections between chromosomal organization, gene-silencing, pseudo-gene localization and horizontal gene transfer. We report the existence of transcriptionally silent contiguous areas corresponding to large regions of H-NS protein binding along the genome, their position indicates a possible relationship with the known large-scale features of chromosome organization.

DOI10.1039/c3mb25364c
Alternate JournalMol Biosyst
PubMed ID23412141