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Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.

TitleMapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.
Publication TypeJournal Article
Year of Publication1996
AuthorsBerger, F, Fischer, G, Kyriacou, A, Decaris, B, Leblond, P
JournalFEMS Microbiol Lett
Volume143
Issue2-3
Pagination167-73
Date Published1996 Oct 1
ISSN0378-1097
KeywordsBase Sequence, Chromosome Mapping, Chromosomes, Bacterial, Cloning, Molecular, DNA Primers, DNA, Bacterial, Genes, Bacterial, Multigene Family, Operon, Polymerase Chain Reaction, RNA, Bacterial, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Streptomyces
Abstract

Streptomyces ambofaciens DSM40697 possess six rRNA gene clusters which can be distinguished by probing BfrI-digested total DNA with the PCR-amplified l6S-23S ribosomal genet internal transcribed spacer. The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA. For five of the six ribosomal gene sets, the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus.

Alternate JournalFEMS Microbiol. Lett.
PubMed ID8837469