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Size control in mammalian cells involves modulation of both growth rate and cell cycle duration.

TitleSize control in mammalian cells involves modulation of both growth rate and cell cycle duration.
Publication TypeJournal Article
Year of Publication2018
AuthorsCadart, C, Monnier, S, Grilli, J, Sáez, PJ, Srivastava, N, Attia, R, Terriac, E, Baum, B, Cosentino Lagomarsino, M, Piel, M
JournalNat Commun
Volume9
Issue1
Pagination3275
Date Published2018 08 16
ISSN2041-1723
Abstract

Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell lines and primary human cells. We find that, in a majority of cell types, the volume added across the cell cycle shows little or no correlation to cell birth size, a homeostatic behavior called "adder". This behavior involves modulation of G1 or S-G2 duration and modulation of growth rate. The precise combination of these mechanisms depends on the cell type and the growth condition. We have developed a mathematical framework to compare size homeostasis in datasets ranging from bacteria to mammalian cells. This reveals that a near-adder behavior is the most common type of size control and highlights the importance of growth rate modulation to size control in mammalian cells.

DOI10.1038/s41467-018-05393-0
Alternate JournalNat Commun
PubMed ID30115907
PubMed Central IDPMC6095894
Grant List311205 PROMICO / / EC | European Research Council (ERC) / International
ANR-14-CE11-0009-03 / / Agence Nationale de la Recherche (French National Research Agency) / International