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Sumoylation of the THO complex regulates the biogenesis of a subset of mRNPs.

TitleSumoylation of the THO complex regulates the biogenesis of a subset of mRNPs.
Publication TypeJournal Article
Year of Publication2014
AuthorsBretes, H, Rouviere, JO, Leger, T, Oeffinger, M, Devaux, F, Doye, V, Palancade, B
JournalNucleic Acids Res
Volume42
Issue8
Pagination5043-58
Date Published2014 Apr
ISSN1362-4962
KeywordsCysteine Endopeptidases, Exosome Multienzyme Ribonuclease Complex, Gene Expression, Nuclear Proteins, Proteome, Ribonucleoproteins, RNA Transport, RNA, Messenger, Saccharomyces cerevisiae Proteins, Stress, Physiological, SUMO-1 Protein, Sumoylation, Ubiquitination
Abstract

Assembly of messenger ribonucleoparticles (mRNPs) is a pivotal step in gene expression, but only a few molecular mechanisms contributing to its regulation have been described. Here, through a comprehensive proteomic survey of mRNP assembly, we demonstrate that the SUMO pathway specifically controls the association of the THO complex with mRNPs. We further show that the THO complex, a key player in the interplay between gene expression, mRNA export and genetic stability, is sumoylated on its Hpr1 subunit and that this modification regulates its association with mRNPs. Altered recruitment of the THO complex onto mRNPs in sumoylation-defective mutants does not affect bulk mRNA export or genetic stability, but impairs the expression of acidic stress-induced genes and, consistently, compromises viability in acidic stress conditions. Importantly, inactivation of the nuclear exosome suppresses the phenotypes of the hpr1 non-sumoylatable mutant, showing that SUMO-dependent mRNP assembly is critical to allow a specific subset of mRNPs to escape degradation. This article thus provides the first example of a SUMO-dependent mRNP-assembly event allowing a refined tuning of gene expression, in particular under specific stress conditions.

DOI10.1093/nar/gku124
Alternate JournalNucleic Acids Res.
PubMed ID24500206
PubMed Central IDPMC4005672