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The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA.

TitleThe unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA.
Publication TypeJournal Article
Year of Publication1996
AuthorsLeblond, P, Fischer, G, Francou, FX, Berger, F, Guerineau, M, Decaris, B
JournalMol Microbiol
Volume19
Issue2
Pagination261-71
Date Published1996 Jan
ISSN0950-382X
KeywordsChromosomes, Bacterial, Deoxyribonucleases, Type II Site-Specific, DNA, Bacterial, Gene Library, Genetic Markers, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Streptomyces
Abstract

Physical maps of the chromosomes of three strains of Streptomyces ambofaciens were constructed by ordering Asel fragments generated from the genomic DNA as a single linear chromosome of about 8 Mb. The physical maps of the three strains were very similar. For strain DSM40697, a Dral map was obtained by positioning the Dral sites relative to the Asel map. Eighteen genetic markers as well as the deletable and amplifiable region were assigned to the Asel and Dral fragments in this strain. The resulting genetic map resembled that of Streptomyces coelicolor A3(2). The two terminal Asel fragments exhibited retarded pulsed-field gel electrophoresis mobility, demonstrating that proteins are covalently bound at this position. A restriction map of this region was made using four additional endonucleases. Repeated sequences present at both ends of the chromosome were mapped as long terminal inverted repeats stretching over 210 kb. This corresponds to the longest terminal inverted repeats so far characterized. The deletable region of S. ambofaciens was localized at the chromosomal extremities.

Alternate JournalMol. Microbiol.
PubMed ID8825772