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Stochasticity and homeostasis in the E. coli replication and division cycle.

TitleStochasticity and homeostasis in the E. coli replication and division cycle.
Publication TypeJournal Article
Year of Publication2015
AuthorsAdiciptaningrum, A, Osella, M, M Moolman, C, Cosentino Lagomarsino, M, Tans, SJ
JournalSci Rep
Volume5
Pagination18261
Date Published2015
ISSN2045-2322
Abstract

How cells correct for stochasticity to coordinate the chromosome replication and cellular division cycle is poorly understood. We used time-lapse microscopy and fluorescently labelled SeqA to determine the timing of birth, initiation, termination, and division, as well as cell size throughout the cell cycle. We found that the time between birth and initiation (B-period) compensates for stochastic variability in birth size and growth rate. The time between termination and division (D-period) also compensates for size and growth variability, invalidating the notion that replication initiation is the principal trigger for cell division. In contrast, the time between initiation and termination (C-period) did not display such compensations. Interestingly, the C-period did show small but systematic decreases for cells that spontaneously grew faster, which suggests a coupling between metabolic fluctuations and replication. An auto-regressive theoretical framework was employed to compare different possible models of sub-period control.

DOI10.1038/srep18261
Alternate JournalSci Rep
PubMed ID26671779
PubMed Central IDPMC4680914